Table 2.
Primers for qPCR
| Gene | Forward primer | Reverse primer |
| dnaE | 5'-AATCACACTCTGCCGCTTATG-3' | 5'-CCATCCGCCAGCACTCAT-3' |
| polA | 5'-GCTGGCTTGCGGATGTAGAT-3' | 5'-AGCACGGCGGTCACTTCA-3' |
| gapA | 5'-CCATCCGTGTTACCGCAGAG-3' | 5'-TCTTAGCACCAGCAGCAATGT-3' |
| fleC | 5'-GTAGTAGCGGTTTTCAGTGCTGT-3 | 5'-CCGGTGGTATTTCAGGTGAT-3' |
| hutI | 5'-ACTCGCAGGTGTCACTTG-3' | 5'-GAGCCAGTAAGCCAATTCAG-3' |
| hutU | 5'-CGATATTCTTCCCAGCTCCA-3' | 5'-GCAACAGATTTTGATGATGCG-3' |
| carA | 5'-AAGGCAGTTGGACTCTGGAA-3' | 5'-CCGTTGGATAAGAAAATGCC-3' |
| carB | 5'-GTTAATTGGCGGCATTATGG-3' | 5'-GACTTCAATCAGGTAAACTTC-3' |
| pyrB | 5'-CCGATTGTCGTTTGAAACCT-3' | 5'-ATTGACCACCGGCACATTGCC-3' |
| deoB | 5'-GCTGACTGAAGGCGGTTACAAT-3' | 5'-GGTGCTGGCGGTTCAACTG-3' |
| acrE | 5'-AGATTCTGGCTAACGACTACG-3' | 5'-ATCACCTGAGCGATGTATGG-3' |
| oppA1 | 5'-TCAAGAACAGCCAGAAGCCCTA-3' | 5'-AAGCGAGCGTGGTTGGAATAC-3' |
| oppB1 | 5'-TGGCGGTGATAGTGGATT-3' | 5'-TGATCTCGGCAATAACTTCC-3' |
| oppC1 | 5'-ACCAAGATCCGTATGTCATT-3' | 5'-TACCGAGCCAGTTAGTGT-3' |
| oppDF | 5'-TGAGATTATCCAGTGCCTTGA-3' | 5'-TAGTCAGAACCGCCATAGC-3' |
| oppB2 | 5'-GCATTCCTACTGGCGGTGATT-3' | 5'-AGGACTAACAGAGGAGCAACGA-3' |
| dppB1 | 5'-CCTTACTGACAGTGATTGC-3' | 5'-ATAATGGCGGTGGTATCG-3' |
| dppB2 | 5'-ACCATTCCTCTCGCCGTTAT-3' | 5'-GACCACAATCACTCGCATCC-3' |
Column 1 presents the genes that were analyzed.
Columns 2 and 3 indicate the primers that were used for the qPCR reaction. Sequence information was obtained from Sanger [22].