Table 1.
Primer sequences for qPCR.
| Putative gene name and symbol | 5'-Forward primer-3' | 5'-Reverse primer-3' | Product size (bp) |
| Genes identified by SSH | |||
| Factor in the germline α (figla) | TCTTGAAGAATGCGATCAACTATGC | CACTCACGTCTCCATCTCCTGC | 177 |
| Zona pellucida protein X (zpx) | TTGTGACACCTTGTTCCGCC | TCGCAACTACACGCCCTTAGAC | 257 |
| Zona pellucida protein C (zpc) | CAGGAAAGCATCCACAGTGAAGC | TTTGTTACGACATCCAATGACCC | 153 |
| Egg envelope glycoprotein (eeg) | TGGTGTCATCTGTCTCTTGAGGC | TTTGAAGCAGTTCTACGGCTCG | 233 |
| Somatic lipoprotein receptor (vldlro) | AACAGAGCAACAACCAGATGCC | TAGAACCAGTATCACGCCCTGC | 278 |
| Cathepsin B, a (ctsba) | GAACACTGACTGGGGAGATAATGG | CTCTGATAGGGTGGAGTTTCCTTTC | 222 |
| Cathepsin D (ctsd) | CGTCATCTTTGACTCCCGATCC | GCACAAGTTTCCATTTGCTTTTCTG | 175 |
| Cathepsin Z (ctsz) | TCCCATCGTTCCCAAAACCTAC | TGTTCCCAAGGCAAAGCACG | 200 |
| Serum lectin isoform 2 (lcal) | CAGGTTCTTATGGAGCACACGG | GTTCAAGCAAAGGTGGAAAAGGAC | 203 |
| Rhamnose binding lectin STL3 (lrham) | ACCAACTCACCGACACCAACTG | CCCAATGGAACACACATCGTG | 290 |
| Alveolin (alv) | ACAGAGAAATCACCTGAGCCCC | GGAGAAATAAAACCACTGCCTGC | 253 |
| Ferritin H-3 (fth3) | ACTCTCCTTCCAGAACAAGCGAG | TTGGTGAGGTTGGTGATGTGG | 264 |
| DnaJ subfamily A member 2 (dnaja2) | TGTCTGCCATCTAAGTGTTTCAACG | CGCTGTAAAAAGTGCGAGGGC | 278 |
| Cyclin E (ccne) | GTTCAAAGGAATCCCAGCAGATG | TCACCGTAGCACAATAAACACCC | 215 |
| Retinol dehydrogenase 1 (rhd1) | GTTCAGGCAGATTCACCACCC | CGATGACCCCGTTCAGGTTC | 210 |
| Coatomer complex protein, epsilon (cope) | CAGATGTCTCCATTGTCTCAAGCAG | TGACCCAGCACTTAGGCAAAGC | 175 |
| Anterior pharynx defective 1b (aph1b) | CTGCTGAAGAAGGCAAACGAAG | AGGAATCAAAGAACACCACACCC | 268 |
| Candidate genes | |||
| Vitellogenin receptor (vldlr) | TCTGGAAGTGTGATGGAGAGAAGG | TCGCTGGGTGAACATTTAGCC | 337 |
| Apolipoprotein E (apoe) | AGGGAGGACATCCAGACCAAGTTG | CGTTTCTTCAGTTTGCGGGTGTAG | 218 |
| Lipoprotein lipase (lpl) | GTGCCTCAACTGCCGTAAGAAC | TGCCCCAAATGTCAGACCAG | 365 |
| FSH receptor (fshr) | GACGCACATCAGAGTGTTTCCC | GTAGAACCCTCAGTCCAGTGTTGC | 242 |
| Anti-Müllerian hormone (amh) | TCACTTTCACCAGTCACTCTCTGC | CACTTCTTGTTCCGTCACCAATC | 204 |
| Gonadal soma-derived growth factor (gsdf) | CCAGAATCAAGAAGGAATACGCAG | TGGGAAAAGAGAGGAGAGCAGG | 317 |
| Elongation factor-1α (ef1a) | CCCCTGGACACAGAGATTTCATC | AGAGTCACACCGTTGGCGTTAC | 409 |