Table 3.
Analysis of total and messenger RNA yields and transcript levels across different stage ovaries of coho salmon (N = 5 fish/stage) in August 2006.
| Parameter | Cohort 1 | Cohort 2 | Cohort 1: mRNA based | Cohort 2: mRNA based | Fold change (mRNA) | Cohort 1: total RNA based | Cohort 2: total RNA based | Fold change (total RNA) |
| Body mass (g) | 77.1 ± 9.5 | 736.5 ± 33.8 | ||||||
| Fork length (cm) | 17.7 ± 0.7 | 36.4 ± 0.9 | ||||||
| Paired ovary mass (g) | 0.22 ± 0.02 | 8.51 ± 0.71 | ||||||
| Gonadosomatic Index (GSI) | 0.30 ± 0.03 | 1.15 ± 0.05 | ||||||
| Follicle stage | late perinucleolus | yolk granule | ||||||
| Est. fecundity (no. of follicles) | 3361 ± 192 | 2999 ± 462 | ||||||
| μg total RNA/g ovary | 4750 ± 80 | 1509 ± 40 | ||||||
| μg total RNA/follicle | 0.31 ± 0.01 | 4.50 ± 0.50 | ||||||
| ng mRNA/μg total RNA | 117.8 ± 4.0 | 23.4 ± 3.2 | ||||||
| ng mRNA/follicle | 36.4 ± 1.6 | 99.5 ± 4.7 | ||||||
| % mRNA | 11.8 ± 0.4 | 2.3 ± 0.3 | ||||||
| Housekeeping gene | ||||||||
| ef1a per template RNA | 1.02 ± 0.01 | 1.18 ± 0.03 | up 1.2 | 1.94 ± 0.05 | 1.33 ± 0.09 | down 1.5 | ||
| ef1a per follicle | 1.14 ± 0.04 | 1.16 ± 0.04 | nc | 5.61 ± 0.15 | 1.24 ± 0.08 | down 4.5 | ||
| Downregulated gene | ||||||||
| fth3 per template RNA | 4.25 ± 0.20 | 1.35 ± 0.13 | down 3.2 | 18.60 ± 0.46 | 1.69 ± 0.26 | down 11.0 | ||
| fth3 per follicle | 4.89 ± 0.20 | 1.38 ± 0.16 | down 3.5 | 58.27 ± 1.99 | 1.69 ± 0.22 | down 34.5 | ||
| fth3/ef1a | 4.69 ± 0.21 | 1.29 ± 0.13 | down 3.6 | 9.81 ± 0.25 | 1.29 ± 0.19 | down 7.6 | ||
| Upregulated gene | ||||||||
| fshr per template RNA | 1.58 ± 0.35 | 12.48 ± 2.53 | up 7.9 | 1.16 ± 0.05 | 3.44 ± 0.38 | up 3.0 | ||
| fshr per follicle | 1.82 ± 0.43 | 12.57 ± 2.70 | up 6.9 | 1.22 ± 0.06 | 1.17 ± 0.12 | nc | ||
| fshr/ef1a | 1.57 ± 0.35 | 10.81 ± 2.41 | up 6.9 | 1.23 ± 0.08 | 5.25 ± 0.34 | up 4.3 |
Cohort 1 fish were brood year 2004 and cohort 2 fish were brood year 2003.
nc, no change