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Neutralization in single cycle infection assays. Wt an d N386D HIV-1LAI virus and wt and N386D pseudovirus derived from HIV-1JR-FL were preincubated with antibody and subsequently incubated with TZM-bl cells containing a luciferase reporter construct under control of the HIV-1 LTR as described in the materials and methods section. The luciferase activity in the absence of inhibiting reagents was set at 100%.
