Figure 3.

Three ASP up-regulated bands and six ASP down-regulated bands were identified by differential display. Northern blot analysis of the differentially displayed bands using total RNAs from melan-a cells (left of each group) and from mouse skin samples (right of each group). Five micrograms of total RNA from cultured melan-a cells, with or without ASP treatment, were blotted to the lanes as marked on the left; 10 μg of total RNA from skins of sibling lethal yellow and black mice are blotted on the right as marked. The same blots reprobed with glyceraldehyde-3-phospho-dehydrogenase (GAPDH) are shown as a loading control.