Fig. 2.

Differential effects of tau phosphorylation by different protein kinases on its ability to stimulate microtubule (MT) assembly. Real-time MT assembly was monitored by measuring the turbidity (O.D._{350 nm}) of tubulin (2.0 mg/mL) at 35 °C in the presence of 0.07 mg/mL control-treated tau₄₄₁ (Con-tau) or tau₄₄₁ phosphorylated by dual-specificity tyrosine-phosphorylated and -regulated kinase 1A (Dyrk1A) (A), glycogen synthase kinase-3β (GSK-3β) (B) or cAMP-dependent protein kinase (PKA) (C). Representative real-time turbidimetric changes of MT assembly of three independent experiments with the same results are shown.