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. 2008 Mar;10(3):244–254. doi: 10.1593/neo.07976

Figure 2.

Figure 2

Nontransformed and transformed prostate epithelial cells express the CXCL5 receptor and endogenously secrete CXCL5. (A) Immunoblot analysis of protein lysates prepared from transformed PC3 and LNCaP, and nontransformed N15C6 and BPH-1 prostate epithelial cells probed with antibodies specific for the CXCL5 receptor, CXCR2, and loading control, β-actin. Primary antibody concentrations used were 1:1000 for CXCR2 and 1:5000 for β-actin. (B) Protein levels (pg/ml) of CXCL5 present in media conditioned by transformed LNCaP and PC3 or nontransformed N15C6 or BPH-1 cells prostate epithelial cells were determined by ELISA. The graph shows the pg/ml CXCL5 detected plotted on a logarithmic scale (y axis).