Table 2. Protein parameters for human topoII isoforms.
Amino acids | Theoretical pI | Acidic amino acids | Basic amino acids | |
TopoIIα | 1–1531 | 8.82 | 226 (14%) | 246 (16%) |
TopoIIβ | 1–1621 | 8.22 | 243 (15%) | 250 (15%) |
Truncated topoIIα | 1–1242 | 8.71 | 173 (14%) | 187 (15%) |
Truncated topoIIβ | 1–1263 | 8.83 | 163 (13%) | 185 (15%) |
α CTD | 1243–1531 | 9.09 | 53 (18%) | 59 (20%) |
β CTD | 1264–1621 | 5.04 | 77 (22%) | 65 (18%) |
TopoIIα +β tail | 1–1600 | 7.69 | 250 (16%) | 252 (16%) |
TopoIIβ+α tail | 1–1552 | 8.9 | 219 (14%) | 244 (16%) |
Shown are the residues of the protein, the theoretical pI and number of acidic (negatively charged, D,E) and basic (positively charged, R,K) amino acids. Shown in parentheses is the percentage of amino acids with each charge in each protein. Reproduced and modified from KL Gilroy, thesis [42].