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. Author manuscript; available in PMC: 2009 Feb 1.

Published in final edited form as: Bioorg Med Chem Lett. 2008 Jan 14;18(3):913–917. doi: 10.1016/j.bmcl.2007.12.048

First derivatives of the UV melting profiles of d[T₂G₃(T₂AG₃)₃A] (profile A) and ST-DNA (profile B) in the absence and presence of macrocyclic lysinyl-containing hexaoxazoles. Profiles were acquired on an AVIV model 14NT-UV-VIS spectrophotometer using quartz cuvettes with a 1 cm pathlength. The temperature was raised in 0.5 °C increments and the samples were allowed to equilibrate for 1.5 minutes at each temperature setting, whereupon absorbances were recorded over a period of 5 seconds and averaged. When present, ST-DNA was used at a base pair concentration of 15 µM, while d[T₂G₃(T₂AG₃)₃A] was used at a strand concentration of 5 µM. Macrocyclic ligands were used at a concentration of 15 µM in the ST-DNA experiments and 20 µM in the d[T₂G₃(T₂AG₃)₃A] experiments. The solution conditions in the ST-DNA experiments were 10 mM EPPS (pH 7.5) and sufficient KCl to bring the total K⁺ concentration to 50 mM. The solution conditions in the d[T₂G₃(T₂AG₃)₃A] experiments were 10 mM potassium phosphate (pH 7.5) and sufficient KCl to bring the total K⁺ concentration to 150 mM. In the ST-DNA experiments, the acquisition wavelength was 260 nm, while being 295 nm in the d[T₂G₃(T₂AG₃)₃A] experiments.

First derivatives of the UV melting profiles of d[T₂G₃(T₂AG₃)₃A] (profile A) and ST-DNA (profile B) in the absence and presence of macrocyclic lysinyl-containing hexaoxazoles. Profiles were acquired on an AVIV model 14NT-UV-VIS spectrophotometer using quartz cuvettes with a 1 cm pathlength. The temperature was raised in 0.5 °C increments and the samples were allowed to equilibrate for 1.5 minutes at each temperature setting, whereupon absorbances were recorded over a period of 5 seconds and averaged. When present, ST-DNA was used at a base pair concentration of 15 µM, while d[T₂G₃(T₂AG₃)₃A] was used at a strand concentration of 5 µM. Macrocyclic ligands were used at a concentration of 15 µM in the ST-DNA experiments and 20 µM in the d[T₂G₃(T₂AG₃)₃A] experiments. The solution conditions in the ST-DNA experiments were 10 mM EPPS (pH 7.5) and sufficient KCl to bring the total K⁺ concentration to 50 mM. The solution conditions in the d[T₂G₃(T₂AG₃)₃A] experiments were 10 mM potassium phosphate (pH 7.5) and sufficient KCl to bring the total K⁺ concentration to 150 mM. In the ST-DNA experiments, the acquisition wavelength was 260 nm, while being 295 nm in the d[T₂G₃(T₂AG₃)₃A] experiments.