Figure 2.

Misregulation of Transcripts Encoding Proteins Involved in T Biosynthesis

A, Expression of genes involved in steroidogenesis in wild-type (white bars) and Ar^{invflox(ex1-neo)/Y} (black bars) testis. Levels of mRNA were normalized between samples based on the levels of Actb in the case of Northern blots and Rps2 for transcripts analyzed by real-time qRT-PCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001 from a two-way ANOVA followed by Bonferroni post test. B, The pathway of T biosynthesis from cholesterol. Red highlights indicate that the transcript was present at significantly higher levels in Ar^{invflox(ex1-neo)/Y} testes at one or more time points during development, whereas green indicates the transcript was present at lower than wild-type levels at one or more time points.