Figure 3.

Expression of Genes Involved in Cholesterol Synthesis and Uptake

Expression of genes involved in cholesterol synthesis and uptake in wild-type (white bars) and Ar^{invflox(ex1-neo)/Y} (black bars) testis. A, Each bar represents the relative mean expression level for the given transcript from six individual animals. Levels of mRNA were normalized between samples based on the levels of Actb in the case of Northern blots and Rps2 for transcripts analyzed by real-time qRT-PCR. All mRNA levels are normalized to the density of Leydig cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001 from a two-way ANOVA followed by Bonferroni post test. B, The pathway of synthesis of cholesterol from acetate.