Figure 1.

RANKL Induces the Synthesis and Expression of Nuclear NFATc1 in RAW264.7 Cells

A, Expression of the murine NFATc1 gene is controlled by two independent promoters P1 and P2. The P1 promoter regulates the synthesis of isoforms A and C, which arise exclusively through the use of alternative polyadenylation sites in the 3′-untranslated region. B, RANKL induces NFATc1 transcripts in RAW264.7 cells. Cells were treated with soluble RANKL (300 ng/ml) for the indicated time periods, and isolated RNA was examined for the presence of β-actin as well as NFATc1 isoforms C, B, and A plus C. C, Expression of NFATc1 in RAW264.7 cells. Cells were treated with sRANKL as above, and the nuclear and cytosolic fractions were subjected to Western blot analysis using antibodies to NFATc1. These data are representative of three independent studies. NT, No treatment.