Figure 1.

Vav3 mRNA Is Elevated in Androgen-Independent LNCaP Xenografts

LNCaP cells and equal volumes of Matrigel were injected sc into male nude mice (n = 13). Mice were left intact or castrated after tumor formation (∼5 wk after inoculation of tumor cells). Approximately 7–10 wk after castration or mock surgeries (intact mice), animals were killed, and tumor tissue was excised and flash frozen. Total RNA was extracted from tumor tissue and LNCaP cells and subjected to semiquantitative RT-PCR analysis using Vav3- and actin-specific primers. Data are plotted as percent of Vav3 mRNA in androgen-dependent (intact) and androgen-independent (castrated) tumor xenografts compared with LNCaP cells ± sem. Graph shows results from four independent RT-PCRs performed in triplicate. Significance was determined using a two-tailed Student’s t test (*, P < 0.05).