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Northern blot analysis of total RNA isolated from control (day 0) and wounded stem tissue of grand fir saplings as a function of the time (day 1–28) after wounding. The blots (20 μg of RNA per gel lane) were probed with a 32P-labeled cDNA fragment of ag1. Equal loading was verified by probing with a fragment of constitutively expressed ubiquitin (data not shown) (15).
