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Immunofluorescent staining for VPAC-1 receptors (Figure 1A) and VIP (Figure 1B) in HepG2 cells. Figure 1C: Negative control, illustrating the specificity of the VPAC-1 and VIP antibodies. VIP and VPAC-1 immunoreactivities were specifically blocked with 10 ug/ml blocking peptides. Figure 1C is reflecting the specificity of VPAC-1 antibody (Blocking of VIP was similar to that of VPAC-1; data is not shown).
