Table 1.
Effect of leptin treatment on carbon source contribution to total hepatic glucose output in ob/ob mouse liver and rate of glycogen synthesis in ob/ob and lean mouse liver
| Group | From endogenous sources | From 13C-pyruvate μmol glucose/glww | Total glucose output | Net rate of glycogen synthesis* μmol per glww/min |
|---|---|---|---|---|
| ob/ob control (7) | 151 ± 12 | 101 ± 8 | 252 ± 11 | 0.36 ± 0.04 |
| ob/ob leptin in vitro (5) | 134 ± 7 | 136 ± 14 | 271 ± 10 | 0.57 ± 0.06† |
| ob/ob leptin in vivo (7) | 115 ± 16 | 216 ± 20‡§ | 331 ± 28¶ | 1.07 ± 0.10‡§ |
| Lean control (3) | 0.84 ± 0.17 | |||
| Lean leptin in vitro (2) | 1.71 ± 0.03 |
Leptin in vivo group received leptin, s.c., 0.5 mg/kg body weight, twice a day for 5 days before liver excision, with no leptin added during perfusion. Livers from the the leptin in vitro group were from untreated mice and received leptin added to the perfusate at a level that matched day 5 serum levels of the leptin in vivo protocol. Control livers received no leptin treatment. Number of experiments is in parentheses.
Rate of incorporation of 13C-labeled glucosyl units/glww per min.
P < 0.05 versus control group.
P < 0.001 versus control group.
P < 0.01 versus leptin in vitro group.
P < 0.01 versus control group.