Figure 11.

BCG-induced increased NF-kappaB activity in J82 human cancer cell line. Human urothelial cancer cell line (J82) was exposed to BCG or saline for 24 hours and the cells were frozen and prepared for ChiP-Q-PCR assays. In this experiment the chromatin was precipitated with a p65 antibody. The final ChIP DNAs were then used as templates for Q-PCR reactions using primer pairs specific for each genomic region of interest. Q-PCR was carried out using Taq polymerase (iQ SYBR Green Supermix, Bio-Rad). Details of the primer sequences and the Genebank accession numbers are given in Table S4 (Additional File 4). Results are presented as "transcription events detected per 1000 cells" for each gene tested. Error bars correspond to standard deviations from the triplicate Q-PCR reactions. Control represents an un-transcribed region of the genome. Asterisks indicate a statistical significant difference (p < 0.05) between saline- and BCG-treated cells.