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Expression of E1 proteins. Western Blot analyses for E1A and 21-kDa E1B proteins expressed in 10 E1-transformed amniocyte cell pools. Cells were lysed and proteins were size fractionated on a SDS-containing polyacrylamide gel. Proteins were transferred to nitrocellulose and probed with anti-E1A or anti-E1B 21-kDa antibody. Protein lysates from HEK293 or primary amniocytes were used as control.
