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Quantitation of hAAT expression in 6 clonal amniocyte cell lines. Single cell cloning was performed from amniocyte cell pools Z171-5A and Z171-5B in passages 26 and 27, respectively. Clonal cells in different passages were plated into 6-well plates, the supernatants were collected after 48 hours and the amounts of secreted hAAT were analysed by ELISA.
