Table 2.
Nucleotide distribution of selected mutants.
| Nucleotide | 964 | 965 | 966 | 967 | 968 | 969 | 970 | 971 |
|---|---|---|---|---|---|---|---|---|
| A. Nucleotide distribution among viable mutants a | ||||||||
| A | 92 (56) | 20 (12) | 46 (28) | 41 (24) | 17 (7) | 67 (50) | 32 (19) | 28 (15) |
| C | 7 (3) | 48 (29) | 14 (3) | 42 (29) | 23 (13) | 5 (0) | 66 (44) | 33 (21) |
| G | 0 | 3 (3) | 40 (32) | 14 (9) | 7 (6) | 34 (15) | 4 (1) | 41 (26) |
| U | 8 (6) | 36 (21) | 7 (2) | 10 (3) | 60 (39) | 1 (0) | 5 (1) | 5 (3) |
| pb | 4.8 × 10−46 | 2.6 × 10−9 | 6.3 × 10−9 | 3.5 × 10−7 | 5.9 × 10−13 | 1.3 × 10−22 | 1.3 × 10−20 | 6.5 × 10−6 |
| |
(1.2 × 10−24) |
(3.5 × 10−5) |
(3.4 × 10−10) |
(4.1 × 10−6) |
(1.3 × 10−9) |
(3.9 × 10−15) |
(1.7 × 10−16) |
(4.1 × 10−4) |
| B. Phylogenetic variation among bacteria | ||||||||
| A | 11690 | 3451 | 140 | 1346 | 9745 | 11676 | 270 | 374 |
| C | 6 | 1507 | 65 | 10095 | 55 | 28 | 11450 | 185 |
| G | 9 | 389 | 10793 | 221 | 44 | 17 | 12 | 11100 |
| U | 19 | 6379 | 729 | 69 | 1886 | 6 | 3 | 70 |
| Deleted | 12 | 10 | 9 | 5 | 6 | 9 | 1 | 7 |
a
Nucleotide distribution in 107 selected mutants.
Numbers in parenthesis indicate the distribution of mutants with >20% function.
The wild-type sequence is in red and underlined.
b
Probability of random distribution based on Chi - square analysis.