FIGURE 4.
Increased basal level but no difference in activation-induced telomerase activity of PBMC and T cells in caregivers. A, Basal PBMC telomerase activity was higher in caregivers compared with controls. Telomerase activity was measured by TRAP assay and normalized to total lymphocytes. B, Basal T cell telomerase activity was higher in caregivers compared with controls. Telomerase activity was measured by TRAP assay. The activity was presented at mean and SEM (n = 12). C, Activation-induced telomerase activity was not significantly different between caregivers and controls. PBMC were stimulated in vitro with anti-CD3 plus anti-CD28 Abs for 72 h and harvested for measurement of telomerase activity. Data are presented as mean and SEM (n = 38); *, p < 0.0001 by Students’ t test.