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. Author manuscript; available in PMC: 2008 Mar 5.
Published in final edited form as: J Immunol. 2007 Oct 1;179(7):4679–4684. doi: 10.4049/jimmunol.179.7.4679

FIGURE 2.

FIGURE 2

The Klf4 gene is deleted in B cells of bKlf4−/− mice. A, Deletion of Klf4 exon2 and exon3 in B cells from bKlf4−/− mice. A 4.8-kb band represents deletion of the Klf4 gene in B cells from bKlf4−/− mice and a 6.9-kb band represents the wild-type Klf4 gene in B cells from bKlf4+/+ mice by Southern blot hybridization. B, Specific deletion of Klf4 in B cells but not T cells from bKlf4−/− mice. Klf4 gene deletion resulted in a 450-bp band only in B cells from bKlf4−/− mice but not in B cells from bKlf4+/+ mice by PCR. In contrast, a 175-bp band can be detected in B and T cells from bKlf4+/+ mice and T cells from bKlf4−/− mice by PCR. C, Loss of Klf4 mRNA expression in B cells from spleen of bKlf4−/− mice by RT-PCR. L32 is a ribosomal protein that served as a control.