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. Author manuscript; available in PMC: 2008 Mar 5.
Published in final edited form as: J Immunol. 2007 Oct 1;179(7):4679–4684. doi: 10.4049/jimmunol.179.7.4679

FIGURE 7.

FIGURE 7

Klf4 directly binds to the promoter of the cyclin D2 gene. A, A diagram of the cyclin D2 gene promoter. The dotted lines cross the promoter region indicate the putative Klf4-binding sites. The arrows indicate the primers used for PCR. B, Klf4 bound specifically to the promoter of cyclin D2. ChIP assay and quantitative PCR were conducted with an anti-Klf4 mAb and an isotype control mAb. Three different regions of the promoter were examined: two putative-binding sites (−336 to −340 and region −626 to −635) showed 2.4- and 2.6-fold higher precipitated DNA by anti-Klf4 Ab than by the control Ab, but no difference in precipitated DNA between two Abs in the region that has no Klf4-putative binding site (−776 to −922). Data were represented as mean ± SEM (n = 3).