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. 2008 Mar 12;3(3):e1786. doi: 10.1371/journal.pone.0001786

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Lane M – markers; lane 1 -processed Bt-CryIIA; lane 2 – unprocessed Bt-CryIIA protoxin; lane 3 - unprocessed Bt-CryIIA protoxin incubated with Mir1-CP for 8 hr; lane 4 – unprocessed Bt-CryIIA protoxin incubated with Mir1-CP for 24 hr; lane 5 – processed Bt-CryIIA toxin incubated with Mir1-CP for 8 hr. Numbers on the left margin refer to the molecular mass of the marker bands in kD. Arrow A indicates the position of the protoxin, arrow B indicates the position of the processed toxin and arrow C marks the position of Mir1-CP (molecular mass of 33 kD) added to the incubation.