Skip to main content
. 2008 Mar 19;3(3):e1808. doi: 10.1371/journal.pone.0001808

Figure 4. MHV-68-induced membrane changes are gp48-dependent.

Figure 4

A. NIH-3T3 cells were infected (1 p.f.u./cell, 16 h) with gp48+ (upper set of images) or gp48− (lower set of images) versions of eGFP-ORF58 MHV-68, then fixed, permeabilized and stained for gp150 (mAb T1A1, left set of images) or gN (mAb 3F7, right set of images). Each mAb was visualized with Alexa568-conjugated goat anti-mouse IgG pAb, eGFP was visualized directly, and nuclei were counterstained with DAPI. The zoomed images correspond to each boxed region above. B. NIH-3T3 cells were infected with ORF27− or ORF27+ eGFP-ORF58 viruses as in A, then visualized by time-lapse confocal microscopy. The boxed region highlights eGFP+ membrane fronds growing away from the ORF27+ infected cell surface. Times are indicated in each panel. The complete image sets make up Movies S6 and S7.