Figure 2.

JRAB/MICAL-L2 is involved in the transport of claudin-1, occludin, and E-cadherin to the PM. (A) MTD-1A cells were transfected with control RNA or JRAB/MICAL-L2 siRNA and subjected to Western blot analysis by using anti-JRAB/MICAL-L2 and anti-β-actin antibodies. The result shown is representative of three independent experiments. (B–D) MTD-1A cells transfected with control RNA or JRAB/MICAL-L2 siRNA were subjected to a Ca²⁺-switch assay and then immunostained with anti-claudin-1, anti-occludin, and anti-E-cadherin antibodies at the indicated time after Ca²⁺ restoration. Representative images of three independent experiments are shown. Bars, 20 μm. Claudin-1, occludin, and E-cadherin length is quantified and shown as the mean and SEM. The asterisks denote a significant difference between control RNA and JRAB/MICAL-L2 siRNA (p < 0.05).