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. 2008 Mar;19(3):971–983. doi: 10.1091/mbc.E07-06-0551

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© 2008 by The American Society for Cell Biology

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Figure 3. — Expression of the Rab13-binding domain of JRAB/MICAL-L2 inhibits the transport of claudin-1, occludin, and E-cadherin to the PM. (A) The Rab13-binding domain of JRAB/MICAL-L2. CH, calponin homology domain; LIM, LIM domain; and CC, coiled-coil domain. (B–D) MTD-1A cells infected with Ad-EGFP (GFP) or Ad-Myc-JRAB/MICAL-L2-C (JRAB-C) were subjected to a Ca²⁺-switch assay, and then they were immunostained with anti-claudin-1, anti-occludin, and anti-E-cadherin antibodies at the indicated time after Ca²⁺ restoration. Representative images of three independent experiments are shown. Bars, 20 μm. Claudin-1, occludin, and E-cadherin length is quantified and shown as the mean and SEM. The asterisks denote a significant difference between GFP and JRAB/MICAL-L2-C (p < 0.05).