Figure 1.

Distribution of Sp1 during the cell cycle. HeLa cells were grown on coverslips in DMEM, fixed using 4% paraformaldehyde, double-labeled with rabbit anti-Sp1 antibodies (G–L) and mouse anti-lamin A/C antibodies (M–R), and then stained with secondary antibodies conjugated with FITC or Cy5, respectively. DNA was stained with DAPI (A–F). Finally, the cells were examined using a confocal laser scanning microscope.