Figure 1.

Substratum detachment induces autophagosome formation in human mammary epithelial cells. (A) MCF-10A cells or 1°HMECs expressing GFP-LC3 were grown attached in full growth media for 48 h (control, left column), starved with Hank's balanced salt solution for 3 h (HBSS, center), or grown detached on poly-HEMA–coated plates in complete growth media for 48 h (suspend, right). Bars, 20 μm. (B) MCF-10A cells grown detached for 24 h were analyzed by transmission electron microscopy. The boxed area in upper panel is enlarged in lower panel. Bars, 200 nm.