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Effect of stable ATG5 or ATG7 depletion on lumen formation. (A and B) Day 20 acini from the indicated cell types grown were immunostained for α-cleaved caspase-3 (green), α-laminin-5 (red), and counterstained with DAPI to detect nuclei (blue). Confocal equatorial cross-sections are shown. Bar, 20 μm.
