Figure 11.
Phosphatidylethanolamine is required for optimal delivery of Fur4p-GFP to the plasma membrane. RY200T [pFl38gF-GFP] cells were grown for 16 h in YPGal medium supplemented (A) with ethanolamine (2 mM), choline (4 mM) or propanolamine (2 mM) and (B) with ethanolamine (250 μM), ethanolamine (50 μM) or ethanolamine (100 μM) + propanolamine (2 mM). Visualization of GFP fluorescence was performed by confocal microscopy. Trans, transmission. (C) RY200T cells were grown as in A, in the presence of the indicated supplements. PE concentrations as nanomoles of FA/109 cells were determined as described in Materials and Methods. Values are means ± S.D. of at least three independent determinations.