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. 2008 Mar;19(3):807–821. doi: 10.1091/mbc.E07-06-0600

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© 2008 by The American Society for Cell Biology

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Figure 5. — Relative impacts of ergosterol and UFA shortage on Fur4p-GFP biogenesis. (A) hem1Δ [pFl38gF-GFP] cells were cultivated to late exponential phase in YPRaff + ALA before shift to fresh YPRaff + ergosterol (+ Erg), YPRaff + Tween 80 (+ Ole), or YPRaff + ergosterol + Tween 80 (+ Erg + Ole). After 7 h under these conditions, Fur4p-GFP synthesis was induced and cellular GFP distribution was observed at the time indicated after induction. (B) After growth as described in A and 180 min after Fur4p-GFP induction, cells were incubated with FM4-64 before visualization by confocal microscopy. hem1Δ end3Δ [pFl38gF-GFP] (C) and hem1Δ rsp5^ts [pFl38gF-GFP] (D) cells were grown as described in Figure 3C. + Erg, YPGal + ergosterol; + Ole, YPGal + Tween 80; and + Erg + Ole, YPGal + ergosterol + Tween 80. Trans, transmission.