Figure 7.

PC species under the different culture conditions. (A) hem1Δ were grown in the media indicated for 7 h, as described in the legend of Figure 6, before lipid extraction and MS analysis of PC species in the positive ion mode. The relative abundance of each molecular PC species was determined as described in Materials and Methods. Values are means + S.D. of at least three independent determinations. The unsaturation ratio (B), the average chain length (C), and the relative percentage of saturated versus monounsaturated and diunsaturated species (D) were calculated from data displayed in A. As an example, PC 32:1 was considered as a monounsaturated specie with an average chain length of 16 carbons.