Figure 3.

Accumulation of centrosome proteins in response to proteasome inhibition does not require microtubules. (A) Control (Ctrl) and PS-341–treated HeLa cells were immunostained for gamma-tubulin (γ-tub) or α-tubulin (α-tub). The first row shows cells with intact microtubules (α-tub), whereas the two bottom rows show cells treated for 6 h with the microtubule inhibitor nocodazole (noco) or the microtubule-stabilizing agent taxol, before proteasome inhibition with PS-341 for an additional 12 h. (B) HeLa cells treated as in A, immunostained for PCM1 and gamma-tubulin (γ-tub). Scale bar, 10 μm.