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. 2008 Mar;19(3):1032–1045. doi: 10.1091/mbc.E07-05-0499

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© 2008 by The American Society for Cell Biology

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Figure 5. — Ypi1 is a nuclear protein. (A) Indirect immunofluorescence with anti-Myc antibody of YPI1–13Myc (JB385-1A) and YPI1-13Myc glc7-129 (JB409-1C) strains. An untagged WT strain (KT1112) serves as the negative control. (B) Immunoblot analysis with anti-Myc antibody of extracts from the YPI1-13Myc (JB385-1A) and GLC7-13Myc (JB412-1B) strains grown to midlog phase in YPD at 30°C. (C) Indirect immunofluorescence of Ypi1-13Myc in WT (JB385-1A) and sds22-6 (JB403-5C) strains at the permissive (24°C) and nonpermissive (37°C) temperatures. (D) Immunoblot analysis of Ypi1-13Myc with anti-Myc antibody of extracts from the strains described in C.