Table 4.

Quantitative analysis of spindle length in large-budded cells after Ypi1 depletion

Strain	% Cells with large buds	% Large-budded cells with spindle length
		0.5–2 μm	>2 μm	Undetermineda
WTb
0 h	32	33	62	4
8 h	31	20	80	0
12 h	34	18	82	0
16 h	36	28	71	3
20 h	42	35	60	5
PGAL1-YPI1
0 h	30	17	79	4
8 h	30	54	44	2
12 h	44	87	10	3
16 h	60	88	5	7
20 h	34	77	13	11
PGAL1-YPI1 mad1Δ
0 h	28	30	63	7
8 h	23	28	67	6
12 h	32	56	42	2
16 h	37	32	55	14
20 h	26	26	71	11
mad1Δ
0 h	27	46	50	4
8 h	38	15	84	1
12 h	40	37	63	0
16 h	42	25	71	3
20 h	41	49	47	5

^a Large-budded cells without a clearly defined spindle were included in this category.

^b The following strains were used: WT, JB289-1A; pGAL-YPI1, JB284-2C; pGAL-YPI1 mad1Δ, JB289-4B; and mad1Δ, JB289-1C.