Skip to main content
. 2008 Mar;19(3):1174–1184. doi: 10.1091/mbc.E07-09-0893

Figure 4.

Figure 4.

The membrane fraction of mboa-7 mutants lacks LPIAT activity. (A and B) In vitro fatty acid incorporation assay using the membrane fractions of wild-type and mboa-7 mutants and different lysophospholipids as acyl acceptors. [14C] EPA was used as an acyl donor. (A) One-dimensional thin layer chromatographic separation of total lipids of the reaction mixture after incubation. FFA, free fatty acid. (B) Each bar represents the mean ± SEM of at least three independent experiments. **p < 0.01. (C) In vitro fatty acid incorporation into lysoPI with different radiolabeled fatty acids as acyl donors. Each bar represents the mean ± SEM of at least three independent experiments. **p < 0.01. (D) Acyl-CoA:lysophospholipid acyltransferase activity in the membrane fractions of wild-type and mboa-7 mutants. [14C]Arachidonoyl-CoA (12.5 μM) and the indicated concentration of lysoPC, lysoPE, or lysoPS were used. (E) [14C]Arachidonoyl-CoA:lysoPI acyltransferase activity in the membrane fractions of wild-type and mboa-7 mutants. [14C]Arachidonoyl-CoA (12.5 μM) and the indicated concentration of lysoPI were used.