Figure 2.

Celastrol simultaneously activates transcription of heat shock and antioxidant genes in yeast. Parallel cultures of wild-type yeast were left untreated (DMSO) or treated with 10 μM celastrol for 1 h, or they were subjected to a 39°C heat shock for 30 min. RNA was isolated and processed for transcriptional profiling by DNA microarray as described in Materials and Methods. Mean induction ratios for each treatment were calculated for each gene, transformed to log₂ and plotted. Selected genes exhibiting significant induction in both treatments are labeled.