Figure 4.

Akt, but not NF-κB, is required for the inhibition of NGF-induced differentiation of SH-SY5Y cells. (A) Effect of inhibitors on NGF-induced β-tubulin III expression. SH-SY5Y cells were cultured with the indicated reagents for 48 h. Inhibitors were added to the culture medium 1 h before NGF addition. (B and C) Effect of exogenously added TNF-α on the expression of β-tubulin III. SH-SY5Y cells were incubated with murine TNF-α (B) or with human TNF-α, with or without the 80M2 mAb (C), in the presence of the Akt inhibitor or the NF-κB inhibitor. After 1-h incubation, NGF was added directly to the culture medium. After 48-h incubation with NGF, cells were fixed.