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. 2008 Mar;19(3):1199–1209. doi: 10.1091/mbc.E07-06-0584

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© 2008 by The American Society for Cell Biology

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Figure 2. — Sgo1 is not required to sense tension at kinetochores during meiosis I. (A–D) Diploid spo11Δ (A2047), spo11Δ mad2Δ (A15500), and spo11Δ pCLB2-SGO1 (A15501) strains each carrying a PDS1–18MYC fusion were sporulated. (A) The percentage of mononucleate (■), binucleate (●), and tetranucleate (□) cells and binucleate cells containing Pds1 (○) was determined at the indicated time points for spo11Δ (top), spo11Δ mad2Δ (middle), and spo11Δ pCLB2-SGO1 (bottom) strains. (B) The percentage of binucleate cells that contain Pds1 is shown. At least 100 cells were counted from the 5-, 6-, and 7-h time points. (C) Examples of Pds1-positive (top) and Pds1-negative (bottom) cells are shown. Pds1 (red), tubulin (Tub; green), and a merged picture with DAPI (blue) are shown. (D) Western blot showing levels of Pds1 throughout the meiotic time course. Pgk1 was used as a loading control.