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. 2008 Mar;19(3):1199–1209. doi: 10.1091/mbc.E07-06-0584

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© 2008 by The American Society for Cell Biology

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Figure 4. — clb1Δ clb4Δ cells lose all cohesin during a single meiotic division. (A) Diagram showing how any possible microtubule–kinetochore attachment will cause tension in cells lacking Mam1. (B–D) Wild-type (A8441) and clb1Δ clb4Δ (A11951) cells, carrying REC8-13MYC and NDC10-6HA fusions were sporulated. (B) The percentage of mononucleate (♦), binucleate (■), and tetranucleated (▲) as well as the sum of binucleate and tetranucleated (□) was determined at the indicated time points. (C) The percentage of mononucleate cells with Rec8 localized to chromatin is shown for wild-type (▩) and clb1Δ clb4Δ (□) strains along with the percentage of binucleate cells with Rec8 colocalizing with kinetochores. Mononucleate cells were counted after 6 h of sporulation, and binucleate cells were counted after 8 h of sporulation. (D) Examples of binucleates with strong, weak, and no Rec8 staining are shown. Rec8 (green), Ndc10 (red), and a merged picture with DAPI (blue).