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. 2008 Mar;19(3):1230–1240. doi: 10.1091/mbc.E07-02-0130

Figure 2.

Figure 2.

Localization of nucleoporins in mitosis. (A) In late prometaphase, the nuclear envelope falls back into the mother cell. Nup214-GFP leaves the envelope (214), whereas Pom152-GFP (152) disperses in the ER, with the most prominent signal in the old nuclear envelope (arrow). Nup107-GFP (107) first distributes into the cytoplasm, but it rapidly reappears in the daughter cell (arrowhead). The outline of the cell is indicated by a profile in the first image. D, daughter cell; M, mother cell. Bar, 3 μm. (B) Average signal intensities of Nup107-RFP and Nup214-RFP within the cytoplasm. Mitotic stages were identified by coexpression of histone 4-GFP (strains FB2N107R_H4G and FB2N214R_H4G). Values are mean ± SD. Sample size is indicated. (C) Average signal intensity of Nup107-GFP on the DNA over background cytoplasmic fluorescence in prometaphase and metaphase. Values are mean ± SD. Sample size is indicated. (D) Line-scan analyses of prometaphase and metaphase cells demonstrate that Nup107 associates with DNA after chromosome migration is completed and cells enter metaphase. The outline of the cells is indicated by a profile. D, daughter cell; and M, mother cell. Bar, 2 μm.