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. 2008 Mar;19(3):1230–1240. doi: 10.1091/mbc.E07-02-0130

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© 2008 by The American Society for Cell Biology

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Figure 4. — Nup107 in U. maydis does not colocalize with kinetochores in mitosis. (A) In metaphase, a short spindle, lableded by YFP-αtubulin (Tub), is formed in the daughter cell that is surrounded by the condensed chromosomes, labeled by histone 4-CFP (H4). Nup107-YFP (107) also encompassed the metaphase spindle. Nup107-RFP (107) does not seem to colocalize with the kinetochore protein Mis12 (Mis), labeled with 3xGFP. Bar, 2 μm. (B) In anaphase A, the spindle (Tub) slowly elongates, and the chromosomes (H4) align along the spindle. Nup107-YFP (107) shows a similar distribution, indicating that the nucleoporin remains at the DNA during early anaphase. Nup107-RFP again does not colocalize with Mis12-3xGFP (Mis).