Table 1.

Unbinding force values (F_u), dissociation constants (K_d), and Gibbs free energies of binding (ΔG) of wild-type and mutant His(H58)Ala

Cys-scFv-fragment	Fu, pN	Kd, nM	ΔG, kJ/mol
Wild type	50  ±  4	0.75	−50.8
Mutant His(H58)Ala	40  ±  3	8.94	−44.8

F_u values with standard deviations are calculated from the means of five histograms.