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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Bioorg Med Chem Lett. 2007 Oct 12;18(1):427–431. doi: 10.1016/j.bmcl.2007.09.104

Table 1.

Peptide sequences tested as substrates and functional inhibitors

Cpda Sequence Sb Ic
1 DABCYL-RRINR-Glu(EDANS)-NH2 + +
2 DABCYL-RRINR-OH + −
3 DABCYL-RRIN-OH + −
4 DABCYL-RRI-OH + −
5 DABCYL-RR-OH − −
6 DABCYL-RRINR-Glu-EDANS + +
7 DABCYL-RRIN-EDANS + +
8 DABCYL-RRI-EDANS + +
a

Compounds 2–5 and DABCYL-Arg-OH have been synthesized and used as LC-MS standards to determine cleavage fragments. Based on retention time and mass detection DABCYL-Arg-OH was one of products in all cleavage mixtures except 5.

b

Substrate: the vertical line designates that the peptides were cleaved by Pla (LC-MS tested).

c

Inhibitor: the inhibitory activity of the peptides was determined using functional assay on fibrin plates.