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a, b, c) Representative PCR results showing the ~800 bp fragment that was amplified. d) A range of template concentrations was tested for TAMV and YOKV. Reactions marked 1 and 2 have 2 μL template RNA, reactions 3 and 4 have 6 μL RNA; reaction 5 has 10 μL template, reactions 2, 4 and 5 have 4 μL of MgSO4. All reactions were performed under identical conditions. (NTC- no template control, L- ladder).
