Table 1.
List of 123 genes identified by SAGE in P. berghei salivary gland sporozoites
| times found | |||||||||
| Tag ID | d14 | d18 | total | GeneDB ID | Known as | Sporozoite expression | Pf orthologue | Predicted Pb/Pf protein domain and/or function | |
| group 1 | tag 335 | 60 | 39 | 99 | PB402615.00.0* | d | Pfgenefinder_63r | ||
| tag 183 | 6 | 89 | 95 | PB100551.00.0 | UIS4 | a, d | -- | SP, TM | |
| tag 190 | 9 | 59 | 68 | PB107461.00.0 | UIS7 | d | -- | TM | |
| tag 269 | 6 | 25 | 31 | PB000374.03.0 | TRAP | d, 1, P | PF13_0201 | SP, vWA-like, TSP1 | |
| tag 226 | 11 | 13 | 24 | PB001063.00.0 | S23 | d, 1 | PF08_0088 | Pb-reticulocyte binding, SP, TM | |
| group 2 | tag 435 | 10 | 7 | 17 | PB000252.01.0 | S13, SPECT2 | d, Pb, 1, P | PFD0430c | SP, MAC/Perforin domain |
| tag 168 | 4 | 12 | 16 | PB000317.00.0* | d, 1, P | PF08_0008 | SP, TM, GPI | ||
| tag 146 | 8 | 8 | 16 | PB000881.01.0 | S21/PbTRSP | d, 1, P | PFA0200w | TM, TSP1 | |
| tag 112 | 11 | 2 | 13 | PB000649.01.0 | ECP1 | Δ, 1, P | PFB0325c | Papain family cysteine protease | |
| tag 110 | 2 | 11 | 13 | PB104017.00.0 | S12 | d | -- | SP | |
| tag 200 | 2 | 9 | 11 | PB000400.01.0* | SIS1 | Δ, 1 | PFE0395c | Pf38, Sexual stage antigen, SP, TM, GPI | |
| tag 75 | 3 | 8 | 11 | PB000484.00.0 | UIS10/PbPL | a | PFF1420w | Phosphatidylcholine-sterol acyltransferase | |
| tag 50 | 4 | 6 | 10 | PB000891.00.0 | Pbs36p | d, 1 | PFD0215c | Pf52, Sexual stage antigen, SP | |
| group 3 | tag 76 | 1 | 8 | 9 | PB104912.00.0* | d, 1 | PF11_0283 | SP, TM | |
| tag 264 | 3 | 5 | 8 | AB125370 | SPECT | d, 1, P | MAL13P1.212 | SP | |
| tag 278 | 4 | 4 | 8 | PB000757.02.0 | SIS2 | Δ, 1, P | PF14_0425 | Fructose-1, 6-bisphosphate aldolase | |
| tag 319 | 1 | 7 | 8 | PB108190.00.0* | UIS1 | a | -- | Serine/Threonine protein kinases, 9 TM | |
| tag 136 | 2 | 5 | 7 | PB100963.00.0 | d | -- | Guanylyl cyclase, TM | ||
| tag 245 | 0 | 6 | 6 | PB000780.01.0* | d, 1 | PFI1230c | 4 TM, asparagine-rich | ||
| tag 530 | 0 | 5 | 5 | PB000264.00.0* | d | PF14_0044 | SP | ||
| tag 118 | 0 | 5 | 5 | PB000411.03.0* | SIS3 | Δ | -- | ||
| tag 294 | 1 | 4 | 5 | PB000464.00.0 | S25 | d, 1, P | PFL0545w | Kinesin-related protein | |
| tag 280 | 0 | 5 | 5 | PB000817.02.0 | d, 1, P | PF08_0054 | Heat shock 70 kDa proteinn | ||
| tag 268 | 1 | 4 | 5 | PB001070.03.0 | d | PFF0685c | Fe-only hydrogenase | ||
| tag 193 | 1 | 4 | 5 | PB101172.00.0 | d | -- | |||
| tag 92 | 2 | 2 | 4 | PB000161.03.0 | d, 1 | PFI0955w | sugar transporter, 6TM | ||
| tag 201 | 0 | 4 | 4 | PB000787.03.0 | SIS4 | Δ, 1 | PFI0320w | Arginase family | |
| tag 101 | 0 | 4 | 4 | PB105090.00.0 | SIS5 | Δ | -- | TM | |
| tag 233 | 1 | 3 | 4 | PB106293.00.0* | d | MAL13P1.365 | SNARE | ||
| tag188 | 2 | 2 | 4 | PB107843.00.0* | SIS6 | Δ, 1 | PF10_0290 | TM | |
| tag 62 | 3 | 1 | 4 | PB108391.00.0 | d, 1, P | PF08_0072 | TM | ||
| tag 276 | 1 | 3 | 4 | PB000632.00.0 | SIS7 | Δ, 1 | PF14_0518 | nifU protein, putative | |
| tag 354 | 3 | 0 | 3 | PB000175.03.0* | SIS8 | Δ, 1 | PFD0915w | TM | |
| tag 171 | 1 | 2 | 3 | PB000416.02.0 | d, 1 | PFD0825c | RNA-binding protein of pumilio/mpt5 family | ||
| tag 326 | 2 | 1 | 3 | PB000422.00.0 | d, 1, P | PF07_0126 | |||
| tag 213 | 0 | 3 | 3 | PB000468.02.0 | SIS9 | Δ, 1 | PFL0335c | Eukaryotic translation initiation factor 5 | |
| tag 197 | 0 | 3 | 3 | PB000626.00.0 | d, 1 | PF14_0224 | PP1-like protein serine/threonine phosphatase | ||
| tag 68 | 1 | 2 | 3 | PB000674.01.0 | SIS10 | Δ, 1, P | PF13_0058 | RNA recognition motif | |
| tag 170 | 0 | 3 | 3 | PB000876.00.0 | Pb, 1, P | MAL8P1.78 | Small heat shock protein | ||
| tag 236 | 0 | 3 | 3 | PB000892.00.0 | Pbs 36 | Δ, 1 | PFD0210c | Pbs36, Sexual stage antigen, PS | |
| tag 20 | 0 | 3 | 3 | PB000911.00.0* | SIS11 | Δ, 1 | PFE0965c | Vacuolar ATP synthetase, 4 TM | |
| tag 8 | 1 | 2 | 3 | PB001101.02.0 | SIS12 | ΔΔ | PF14_0395 | ||
| tag 119 | 0 | 3 | 3 | PB001143.02.0 | SIS13 | Δ, 1, P | PF14_0083 | Ribosomal protein S8e | |
| tag 122 | 1 | 2 | 3 | PB001247.02.0 | S14 | d, 1, P | PFL0370w | ||
| tag 127 | 0 | 3 | 3 | PB001432.02.0 | SIS14 | Δ, 1 | PFE0340c | Peptidase S54, rhomboid, 7 TM | |
| tag 155 | 3 | 0 | 3 | PB001575.02.0 | SIS15 | Δ, 1 | PFB0270w | SP, TM, Fe-S metabolism associated domain | |
| tag 150 | 0 | 3 | 3 | PB104165.00.0 | d, 1 | PFI0675w | |||
| tag 305 | 0 | 3 | 3 | PB104461.00.0 | d | -- | SP, TM | ||
| tag 350 | 0 | 3 | 3 | PB105607.00.0 | d | -- | 2 TM | ||
| tag 106 | 1 | 2 | 3 | PB105732.00.0 | d | -- | TM | ||
| tag 196 | 0 | 3 | 3 | PB106033.00.0 | SIS16 | Δ, 1 | PF10_0296 | ||
| tag 41 | 1 | 2 | 3 | PB107193.00.0* | SIS17 | ΔΔ | PFF1195c | ||
| tag 121 | 1 | 2 | 3 | PB108098.00.0 | d | -- | TM | ||
| tag 157 | 2 | 1 | 3 | PB300784.00.0 | d, 1 | MAL8P1.154 | |||
| tag 362 | 0 | 2 | 2 | PY01277** | SIS18 | ΔΔ | PFL2080c | ||
| tag 142 | 0 | 2 | 2 | PB000006.00.0 | SIS19 | Δ, 1 | PFE0995c | ||
| tag 265 | 0 | 2 | 2 | PB000064.03.0 | SIS20 | ΔΔ | PFB0510w | 3 TM | |
| tag 143 | 0 | 2 | 2 | PB000092.02.0 | UIS2 | a, 1, P | PF14_0614 | ||
| tag 355 | 1 | 1 | 2 | PB000094.03.0 | SIS21 | Δ, 1, P | MAL12P1.180 | Arginyl-tRNA synthetase | |
| tag 15 | 1 | 1 | 2 | PB000111.01.0 | d, 1, P | MAL12P1.63 | |||
| tag 57 | 1 | 1 | 2 | PB000120.01.0 | SIS22 | Δ, P | PFC1065w | ||
| tag 26 | 1 | 1 | 2 | PB000133.00.0 | SIS23 | Δ | -- | ||
| tag 59 | 0 | 2 | 2 | PB000171.01.0 | SIS24 | Δ | -- | ||
| tag 339 | 0 | 2 | 2 | PB000178.02.0 | SIS25 | Δ, 1 | PFE0810c | 40S ribosomal subunit protein S14 | |
| tag 139 | 0 | 2 | 2 | PB000207.01.0 | SIS26 | Δ, 1 | PFL1050w | SP, TRAF-like | |
| tag 221 | 0 | 2 | 2 | PB000297.01.0 | SIS27 | Δ, 1 | PF11_0251 | SP, Endoplasmic reticulum oxidoreductin | |
| tag 12 | 0 | 2 | 2 | PB000307.01.0 | UIS9-7 | a, 1 | PFE0935c | RNA-binding protein | |
| tag 134 | 0 | 2 | 2 | PB000352.00.0 | SIS28 | Δ, 1 | PF11_0150 | SP, 6TM, Rhomboid family, SecY protein | |
| tag 51 | 2 | 0 | 2 | PB000352.02.0 | d, 1, P | PFL2440w | DNA repair protein rhp16 | ||
| tag 317 | 1 | 1 | 2 | PB000354.03.0 | d, 1 | PF10_0283 | TM | ||
| tag 285 | 0 | 2 | 2 | PB000396.01.0 | SIS29 | Δ, 1 | PFC0330w | PDZ domain protein | |
| tag 292 | 0 | 2 | 2 | PB000412.02.0 | SIS30 | Δ, 1 | PFI0750c | ||
| tag 17 | 0 | 2 | 2 | PB000424.02.0 | d, 1 | PF11_0358 | DNA-directed RNA polymerase, beta subunit | ||
| tag 320 | 0 | 2 | 2 | PB000450.03.0 | SIS31 | Δ, 1 | MAL13P1.148 | myosin | |
| tag 54 | 0 | 2 | 2 | PB000530.00.0 | d, 1 | PFE0400w | ankyrin | ||
| tag 277 | 0 | 2 | 2 | PB000553.03.0 | d, 1 | PF13_0161 | |||
| tag 303 | 1 | 1 | 2 | PB000591.00.0 | SIS32 | ΔΔ | PFC0125w | DNAJ domain protein | |
| tag 184 | 0 | 2 | 2 | PB000599.03.0 | SIS33 | Δ, 1 | PF14_0681 | Diacylglycerol kinase | |
| tag 34 | 0 | 2 | 2 | PB000604.01.0 | d | PFF0840w | |||
| tag 87 | 0 | 2 | 2 | PB000610.02.0 | SIS34 | Δ, 1 | PFL2390c | ||
| tag 204 | 0 | 2 | 2 | PB000624.01.0 | SIS35 | Δ, 1 | PF13_0261 | ATP binding protein | |
| tag 128 | 0 | 2 | 2 | PB000650.00.0 | SIS36 | Δ, 1, P | PF14_0446 | Fasciclin domain | |
| tag 58 | 0 | 2 | 2 | PB000675.02.0 | SIS37 | ΔΔ | PFF1450w | Sec14-like cytosolic factor | |
| tag 144 | 1 | 1 | 2 | PB000709.00.0 | SIS38 | Δ, 1 | PF14_0181 | Calmodulin | |
| tag 289 | 2 | 0 | 2 | PB000798.02.0 | MAEBL | d, 1, P | PF11_0486 | MAEBL, SP, TM | |
| tag 88 | 0 | 2 | 2 | PB000807.01.0 | SIS39 | Δ, 1 | PFD0485w | Ferlin like protein | |
| tag 353 | 0 | 2 | 2 | PB000811.01.0 | d, 1 | MAL7P1.125 | |||
| tag 80 | 1 | 1 | 2 | PB000837.02.0 | SIS40 | Δ, 1 | PF14_0695 | DNA-directed RNA polymerase, alpha subunit | |
| tag 116 | 0 | 2 | 2 | PB000857.01.0 | SIS41 | Δ, 1, P | PF11_0183 | GTP-binding nuclear protein ran/tc4 | |
| tag 238 | 0 | 2 | 2 | PB000874.02.0 | SIS42 | Δ, 1 | MAL8P1.142 | Proteasome beta-subunit | |
| tag 79 | 0 | 2 | 2 | PB000903.01.0 | SIS43 | Δ, 1 | PF11_0197 | SP, Ankyrin repeat | |
| tag 227 | 0 | 2 | 2 | PB000904.01.0 | SIS44 | Δ, 1 | PF11_0196 | SP | |
| tag 206 | 0 | 2 | 2 | PB000927.03.0 | SIS45 | Δ, 1 | MAL12P1.113 | Heat shock protein DNAJ homologue Pfj4 | |
| tag 210 | 0 | 2 | 2 | PB000941.01.0 | SIS46 | Δ, 1 | PF10_0096 | Octapeptide repeat | |
| tag 349 | 0 | 2 | 2 | PB000994.02.0* | d, 1 | PFL1690w | |||
| tag 218 | 0 | 2 | 2 | PB001066.02.0 | d, 1 | PF10_0140 | SPRY | ||
| tag 228 | 0 | 2 | 2 | PB001080.02.0 | SIS47 | Δ, 1 | PFL0870w | SP, TM, TSP, thrombospondin-related 3 | |
| tag 267 | 1 | 1 | 2 | PB001118.02.0 | UIS4-6 | d, 1 | PFA0170c | TM | |
| tag 27 | 0 | 2 | 2 | PB001120.00.0 | SIS48 | Δ, 1 | MAL13P1.150 | 2 TM | |
| tag 6 | 0 | 2 | 2 | PB001130.00.0 | SIS49 | Δ, 1, P | MAL8P1.133 | Glycosyltransferase family 28 protein | |
| tag 124 | 1 | 1 | 2 | PB001157.02.0 | SIS50 | Δ, 1 | MAL8P1.67 | PIN domain like | |
| tag 301 | 0 | 2 | 2 | PB001178.02.0 | SIS51 | Δ, 1 | PFD0300w | ||
| tag 123 | 0 | 2 | 2 | PB001253.00.0 | SIS52 | Δ, 1 | PFD0360w | Zinc finger | |
| tag 331 | 0 | 2 | 2 | PB001305.00.0 | SIS53 | Δ, 1, P | PF11_0168 | Serine esterase | |
| tag 70 | 1 | 1 | 2 | PB001374.02.0 | d, 1, P | MAL13P1.304 | Malaria antigen, metalloendopeptidase | ||
| tag 31 | 0 | 2 | 2 | PB001507.02.0 | SIS54 | ΔΔ | PFB0620w | ||
| tag 312 | 0 | 2 | 2 | PB001517.02.0 | d | PFF0800w | SP, TM, vWFA, TSP1 | ||
| tag 43 | 1 | 1 | 2 | PB100341.00.0 | SIS55 | Δ | -- | ||
| tag 93 | 1 | 1 | 2 | PB101083.00.0 | d | -- | |||
| tag 61 | 2 | 0 | 2 | PB101148.00.0 | SIS56 | Δ | -- | ||
| tag 114 | 2 | 0 | 2 | PB101408.00.0 | SIS57 | Δ, 1 | PFE1260c | TM | |
| tag 56 | 2 | 0 | 2 | PB101596.00.0 | SIS58 | Δ | -- | ||
| tag 342 | 0 | 2 | 2 | PB102211.00.0 | SIS59 | Δ | -- | ||
| tag 318 | 1 | 1 | 2 | PB102277.00.0 | d | -- | |||
| tag 71 | 0 | 2 | 2 | PB103980.00.0 | SIS60 | Δ | -- | 2 TM | |
| tag 271 | 0 | 2 | 2 | PB105487.00.0 | d | -- | 2 TM | ||
| tag 35 | 0 | 2 | 2 | PB106484.00.0 | SIS61 | Δ, 1 | PF14_0449 | ||
| tag 208 | 0 | 2 | 2 | PB108086.00.0 | SIS62 | Δ | -- | ||
| tag 151 | 0 | 2 | 2 | PB000618.02.0 | SIS63 | Δ | PFD0725c | arsenical pump-driving ATPase, putative | |
| tag 179 | 1 | 1 | 2 | PB300567.00.0 | SIS64 | ΔΔ | PFL2420w | ||
| tag 85 | 0 | 2 | 2 | PB300953.00.0 | SIS65 | ΔΔ | MAL8P1.121 | ||
| tag 33 | 1 | 1 | 2 | PB301273.00.0 | d, 1 | PFL1695c | |||
| tag 47 | 0 | 2 | 2 | PB000460.01.0 | SIS66 | Δ, 1 | PFC0750w | SP | |
(a) : mRNA detected in P. berghei sporozoites [14].
(d): mRNA detected in P. yoelii sporozoites [13].
Pb : detected by proteomics in P. berghei sporozoites [11].
P : detected by proteomics in P. falciparum sporozoites [10].
1 : detected in P. falciparum sporozoites with microarrays [9].
SP : signal peptide.
TM : transmembrane domain.
** : orthologue of a P. yoelli gene for which a P. berghei gene is not yet annotated.
SIS: new Sporozoite expressed gene Identified by SAGE, this work.
Δ : no rodent Plasmodium sporozoite ESTs or proteomics data.
ΔΔ : no sporozoite ESTs or detection by microarrays or proteomics in Plasmodium species.