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. 2008 Jan 24;7:19. doi: 10.1186/1475-2875-7-19

Figure 3.

Figure 3

Localization of PfK1 and PfK2. Indirect Immunofluorescence Assays (IFAs) were performed using affinity purified anti-PfK1 F4 and -PfK2 F4 antibodies, and anti-KAHRP and anti-MESA antisera. Representative IFAs are shown for each antibody. A Anti.-PfK1 IFAs showed no labeling in rings (data not shown) or uRBCs, and increasing RBC membrane labeling in trophozoites and schizonts. Predominantly labeling occurs in a semi-punctate localization pattern. B. PfK2 was not detected in rings (data not shown) or uRBCs, and minimal levels were detected in trophozoites. Schizonts showed intense foci of labeling, which appeared to correspond with the location of the developing merozoites within RBCs. C. KAHRP was detected in a classic punctuate pattern on the surface of trophozoites and schizonts. No labeling of KAHRP was detected in ring-iRBCs (data not shown). D. More diffuse RBC membrane labeling of MESA was detected on trophozoites and schizonts. No labeling was detected in rings (data not shown).