Table 1.
Oligonucleotide primers.
| Primer | Sequence (5'→ 3')a | Targetb |
| P1 | cgggaattcATGAATAATGATAATATTGGGAG | pfk1 full length (+) |
| P2 | cccaagcttTCAGACTTGGTCATGCGGTTC | pfk1 full length (-) |
| P3 | cgggaattcGTTGATAAGAAAGGAAAAATAATAGAT | pfk1 F2 (+) |
| P4 | cccaagcttATGTACAAATCGATGTTTTGTATA | pfk1 F2 (-) |
| P5 | ccgggaattcatTTTAATAATCATGATCATCAGAATC | pfk1 F3 (+) |
| P6 | cccaagcttTAAACATTCTTGATCATCTTTTTCC | pfk1 F3 (-) |
| P7 | ccgggaattctcAAAAAAGGACAAAACCATCAACTT | pfk1 F4 (+) |
| P8 | cccaagcttCATATTTTTTTCCATATCTTTAATG | pfk1 F4 (-) |
| P9 | cgcggatccgATGAAAAGCGGATTATTTTCTATG | pfk2 full length (+) |
| P10 | ccggaattcTCACAATATATAAACTATATCATCGAATC | pfk2 full length (-) |
| P11 | ccgggaattcATAATGATAATGATAATAATAATATTATA | pfk2 F3 (+) |
| P12 | cccaagctttTTTAATTCCTTTTTATTATCAATTGT | pfk2 F3 (-) |
| P13 | ccgggaattcGAATATCAAGAATATTTACCAAAAAC | pfk2 F4 (+) |
| P14 | cccaagcttATTATGAATATGAATATTTGTATTTGA | pfk2 F4 (-) |
| P15 | aaactgcagTGAAAACAGAATGCGATTAGTTCG | pfk1 KO (+) |
| P16 | cgcggatccGATGATGCAAATGTTAATGGTAC | pfk1 KO (-) |
| P17 | aaactgcagTTCTTATCTTATTGAACGGATCTC | pfk2 KO (+) |
| P18 | cgcggatccTTGTATGGTATATATAGTAGATGC | pfk2 KO (-) |
| P19 | GATAGCGATTTTTTTTACTGTCTG | hrp2 3'UTR (+) |
| P20 | GAAATTAACCCTCACTAAAGGG | plasmid backbone |
| P21 | aaggaaaaaagcggccgcAATGAATAATGATAATATTGGGAG | pfk1 (+) |
| P22 | cccaagcttAGATATAATACCATCCGTTTGG | pfk1 (-) |
| P23 | ccggaattcAATGAAAAGCGGATTATTTTCTATG | pfk2 (+) |
| P24 | cgcggatccTTGTATGGTATATATAGTAGATGC | pfk2 (-) |
| P25 | TTGTTGTGGTTCTACATCTCC | pfk2 (-) |
| P26 | CACGAAGCCGCCACACATTGCC | hrp2 orf1 |
| P27 | TTTGATCTTGTTCACTATGGC | pfk1 (-) |
| P28 | cccaagcttATTACAAAAATAATAATTGAAATTACT | pfk2 (-) |
| P29 | GTAATACGACTCACTATAGGGC | plasmid backbone |
| P30 | TTGCAATTCTGCTTCAGTTGG | cam (-) |
a Sequences shown in upper case are gene specific. Underlined sequences indicate enzyme restriction sites.
b + and - indicate sense and anti-sense strand sequences, respectively.