Figure 3.

HRV14 digestion with and without Win 52084 drug present. The MALDI-MS mass analysis was performed on HRV14 alone before digestion (A), after digestion of the HRV14/drug mixture (B), and after digestion of HRV14 alone (no drug present) (C). Digestions shown were performed for 3 h. It is important to note that in each case the virus was exposed for the same amount of time and the same concentration of enzyme; yet the drug-exposed virus was extremely resistant to digestion. Control experiments with FHV clearly demonstrated that the drug was not inhibiting the enzyme.